Intitulé du poste : Post-doctorant (on line September 1st, 2010)
Type de contrat : CDD
Durée : 2 ans
Structure : IMN BORDEAUX, FRANCE
Niveau de diplôme requis : > thèse
Intitulé du poste : Post-Doctorant en neurophysiologie
Fourchette de salaire : 1800 - 2200€ net mensuels, en fonction de l'expérience

Level: PhD
title: Neurophysiology fellowship
Range of salary : 1800 - 2200€ per month (net) (according to experience)
Host Organization :
The « Institut des Maladies Neurodégénératives » (IMN – Institute of Neurodegenerative Disorders) is a research unit under the guardianship of the CNRS and the University Victor Segalen-Bordeaux 2. It supports close links with the University Hospital of Bordeaux. The laboratory, featuring 100 persons, is an integral part of the Neurocampus, the Bordeaux Excellence center for Neuroscience. The laboratory is organized into 7 independent scientific teams.

STRUCTURE : L'institut des maladies neurodégénératives est une unité mixte Université de Bordeaux 2- CNRS qui fait partie du Neurocampus de Bordeaux. Cet institut, qui se compose d'une 100' de personnes, est composé de 7 équipes de recherche en neurosciences.
Position profile : Post-doctoral researcher (m/f)
Duration : 2 years contract
Structure : IMN BORDEAUX, FRANCE

MISSION :
The post-doc will be in charge, under the supervision of the project leader and in close collaboration with the other project members, of a project aiming at restoring motor performances in the Parkinson's disease gold standard, the MPTP-treated non-human primate, through the development of brain-machine interface.
This project is supported by European contract (NEUWALK) aiming at characterizing the electrophysiologial and cinematic parameters of a "normal" state, at developing the movement analysis methodology, at performing the surgical implantation of a "smart" stimulator invented and developed by our partners in the European network on the parkinsonian monkeys and at quantifying the behavioral consequences.

ACTVITIES :
To develop protocols and adapt the experimental conditions in order to answer to the project goals
To jointly run electrophysiological investigations (chronic EMG recordings, tridimensional kinematics, cortical and subcortical multi-single unit telemetric recordings) in a pathophysiological context (macaque model of Parkinson's disease)
To share and exploit the data with the European partners in order to better define and improve the characteristics of the spinal stimulator.

EXPERIENCE :
Strong knowledge in movement physiology, Basal Ganglia physiology, and cellular biology.
Proven practice in electrophysiology – Master the conceptual framework
Fluent in English

ABILITY :
The following attributes will be well-regarded: ability to communicate with people at all levels, strong time organization proficiency, aptitude to work both unsupervised and as a part of a team, competency in data processing and in using analysis softwares (e.g. Mathlab).

Contact :
To apply, send a e-mail at Erwan Bezard (Erwan.bezard@u-bordeaux2.fr) and Christian Gross (christian.gross@u-bordeaux2.fr), with a cover letter, a CV, the list of publications and the summary of the PhD dissertation

Development of a new technique for fast multiphoton super-resolution imaging of thick biological samples

Project:
The teams “Dynamic of receptor organization in neuronal membranes” and “Quantitative Imaging of the Cell” at the laboratory Cellular Physiology of the Synapse in Bordeaux (France) have one position opening for a PhD student.
The PhD project is developed in the framework of European network, SyMBaD (Synapse: from Molecules to higher Brain function And Diseases) and in partnership with an industrial collaborator. The aim is to set up an innovative fast multiphoton imaging technique which will allow imaging of thick biological samples at the single molecule level. This technique will be applied to study the movement of receptors in live neurons, in brain slices. The setup will be designed to be versatile in order to implement, on the same microscope, multiphoton SPT-PALM (Single Particle Tracking & PhotoActivation Localization Microscopy) and SIM (Structured Illumination Microscopy) techniques.

Scientific Environment:
The “Quantitative Imaging of the Cell” is a newly created team, headed by Jean-Baptiste Sibarita, which develops new advanced cellular imaging techniques with the goal to monitor the dynamic of biological compartments at high spatial and temporal resolutions. Other related projects also being developed are FRAP (Fluorescence Recovery After Photobleaching), SPT-PALM (in 2 and 3 dimensions) and SIM. In addition to instrumental development, the team also develops software solutions for image processing and analysis, as well as instrumentation for micropatterning and microfluidics to control cell shape and chemical environment.
The team “Dynamic of receptor organization in neuronal membranes”, headed by Daniel Choquet, aims at understanding the role of glutamate receptor trafficking in controlling synaptic transmission in health and disease. It has developed and applied in the last decade a series of single molecule tracking approaches to image receptor diffusion in live neurons (e.g. Borgdorff & Choquet, Nature, 2002; Triller and Choquet, Neuron, 2008). Recently, the team has associated imaging and electrophysiological approaches to uncover a new unsuspected role of glutamate receptor fast diffusion in controlling information processing in the brain (Heine & al., Science 2008). The next challenge lies in imaging receptor trafficking in 3D in brain slices and to associate this with the analysis of receptor trafficking in vivo.
The industrial collaborator is Amplitude Systèmes, a French company which develops femtosecond oscillator for life science.

Environment: Bordeaux is located in South West of France (3h from Paris by train). This charming town, near the Atlantic Ocean, has a city center which is world heritage of the UNESCO.

Qualification:
The candidate should be a non French citizen, and preferentially be originally from one of the European Union countries. He or she should have a physics background and a strong interest in the treatment of biological questions. Important for work in the lab is the ability to build optical instrumentation, and to write programs that control the setup and automate the data acquisition. Masters or equivalent degree is required.

To apply, send login to the website http://symbad.risc.cnrs.fr/ and register
In parallel, please send a complete CV, a motivation letter, and the names and addresses of two referees for letters of recommendation to:
Daniel Choquet, daniel.choquet@u-bordeaux2.fr
Jean-Baptiste Sibarita, jean-baptiste.sibarita@u-bordeaux2.fr

Institut Magendie – UMR 5091 CNRS, Université Bordeaux 2, 146, rue Léo Saignat
33077 Bordeaux cedex, For further details on the Cellular Physiology of the Synapse, please visit our website: http://www.synapse.u-bordeaux2.fr/equipechoquet.htm