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1847 references
Giannone G, Hosy E, Levet F, Constals A, Schulze K, Sobolevsky AI, Rosconi MP, Gouaux E, Tampé R, Choquet D, Cognet L. Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density. Biophys J. 2010 Aug 9;99(4):1303-10. 2010
IF : 4.390
Liens vers résumé
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| Lab |
Physiologie cellulaire de la synapse - Christophe Mulle |
| Team |
Dynamique de l'organisation membranaire des récepteurs - Daniel Choquet |
| Program areas |
Synapse |
| National Collaboration |
- Centre de Physique Moléculaire Optique et Hertzienne, UMR 5798, Centre National de la Recherche Scientifique, Talence, France
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| European Collaboration |
- Institute of Biochemistry, Biocenter, Goethe University-Frankfurt, Frankfurt, Germany |
| International Collaboration |
- Vollum Institute, Oregon Health and Science University, Portland, Oregon
- Howard Hughes Medical Institute, Oregon Health and Science University, Portland, Oregon
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| Abstract |
Versatile superresolution imaging methods, able to give dynamic information of endogenous molecules at high density, are still lacking in biological science. Here, superresolved images and diffusion maps of membrane proteins are obtained on living cells. The method consists of recording thousands of single-molecule trajectories that appear sequentially on a cell surface upon continuously labeling molecules of interest. It allows studying any molecules that can be labeled with fluorescent ligands including endogenous membrane proteins on living cells. This approach, named universal PAINT (uPAINT), generalizes the previously developed point-accumulation-for-imaging-in-nanoscale-topography (PAINT) method for dynamic imaging of arbitrary membrane biomolecules. We show here that the unprecedented large statistics obtained by uPAINT on single cells reveal local diffusion properties of specific proteins, either in distinct membrane compartments of adherent cells or in neuronal synapses. |
| Principal Publication |
oui |
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Opazo P, Labrecque S, Tigaret CM, Frouin A, Wiseman PW, De Koninck P, Choquet D. CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin. Neuron. 2010 Jul 29;67(2):239-52. 2010
original article. IF : 13.260
Liens vers résumé
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| Lab |
Physiologie cellulaire de la synapse - Christophe Mulle |
| Team |
Dynamique de l'organisation membranaire des récepteurs - Daniel Choquet |
| Program areas |
Synapse |
| International Collaboration |
- Centre de Recherche Université Laval Robert-Giffard, Québec, Canada G1J 2G3
- Departments of Physics and Chemistry, McGill University, Montréal, QC, Canada H3A2T8 |
| Abstract |
The Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is critically required for the synaptic recruitment of AMPA-type glutamate receptors (AMPARs) during both development and plasticity. However, the underlying mechanism is unknown. Using single-particle tracking of AMPARs, we show that CaMKII activation and postsynaptic translocation induce the synaptic trapping of AMPARs diffusing in the membrane. AMPAR immobilization requires both phosphorylation of the auxiliary subunit Stargazin and its binding to PDZ domain scaffolds. It does not depend on the PDZ binding domain of GluA1 AMPAR subunit nor its phosphorylation at Ser831. Finally, CaMKII-dependent AMPAR immobilization regulates short-term plasticity. Thus, NMDA-dependent Ca(2+) influx in the post-synapse triggers a CaMKII- and Stargazin-dependent decrease in AMPAR diffusional exchange at synapses that controls synaptic function. |
| Principal Publication |
oui |
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Carlton PM, Boulanger J, Kervrann C, Sibarita JB, Salamero J, Gordon-Messer S, Bressan D, Haber JE, Haase S, Shao L, Winoto L, Matsuda A, Kner P, Uzawa S, Gustafsson M, Kam Z, Agard DA, Sedat JW. Inaugural Article: Fast live simultaneous multiwavelength four-dimensional optical microscopy. Proc Natl Acad Sci USA. 2010 Aug 12. 2010
IF : 9.432
Liens vers résumé
Fulltext PDF |
| Lab |
Physiologie cellulaire de la synapse - Christophe Mulle |
| Team |
Imagerie quantitative de la cellule - Jean-Baptiste Sibarita - |
| Program areas |
Synapse |
| National Collaboration |
- Institut Curie, Centre de Recherche, 12 rue Lhomond, F-75005 Paris, France;
- Institut National de Recherche en Informatique et en Automatique (INRIA) Rennes—Bretagne Atlantique, Campus Universitaire de Beaulieu, F-35042 Rennes
- Institut National de la Recherche Agronomique (INRA), UR341 Mathématiques et Informatique Appliquées, F-78352 Jouy-en-Josas, France;
- Institut Curie, Plate-forme Imagerie Cellulaire et Tissulaire - Infrastructures en Biologie Sante et Agronomie (PICT-IBiSA) UMR 144, 26 rue d’Ulm 75248 Paris cedex 05, France |
| European Collaboration |
- Institute for Experimental Physics, WE 1, Department of Physics, Free University, Berlin, Arnimallee 14, D-14195 Berlin, Germany |
| International Collaboration |
- The Keck Center for Advanced Microscopy and the Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158-2517;
- Rosenstiel Center and Department of Biology, Brandeis University, 415 South St, Waltham, MA 02454-9110;
- Howard Hughes Medical Institute and Department of Molecular and Cellular Biology, Genetics and Development, University of California, Berkeley, CA 94720-3204;
- Weizmann Institute of Science, Molecular Cell Biology, Rehovot, Israel 76100; and
- The Keck Center for Advanced Microscopy, Howard Hughes Medical Institute and the Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158 |
| Abstract |
Live fluorescence microscopy has the unique capability to probe dynamic processes, linking molecular components and their localization with function. A key goal of microscopy is to increase spatial and temporal resolution while simultaneously permitting identification of multiple specific components. We demonstrate a new microscope platform, OMX, that enables subsecond, multicolor four-dimensional data acquisition and also provides access to subdiffraction structured illumination imaging. Using this platform to image chromosome movement during a complete yeast cell cycle at one 3D image stack per second reveals an unexpected degree of photosensitivity of fluorophore-containing cells. To avoid perturbation of cell division, excitation levels had to be attenuated between 100 and 10,000x below the level normally used for imaging. We show that an image denoising algorithm that exploits redundancy in the image sequence over space and time allows recovery of biological information from the low light level noisy images while maintaining full cell viability with no fading. |
| Principal Publication |
oui |
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Angst J, Cui L, Swendsen J, Rothen S, Cravchik A, Kessler RC, Merikangas KR. Major Depressive Disorder With Subthreshold Bipolarity in the National Comorbidity Survey Replication. Am J Psychiatry. 2010 Aug 16. 2010
original article. IF : 12.522
Liens vers résumé
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| Lab |
Imagerie moléculaire et fonctionnelle : de la physiologie à la thérapie - Chrit Moonen |
| Team |
Neurosciences cognitives humaines et neuro-imagerie - Michèle Allard |
| Program areas |
Fonctions cognitives normales et pathologiques |
| National Collaboration |
the Department of Health Care Policy, Harvard Medical School, Boston UK |
| International Collaboration |
- Department of Psychiatry, Zurich University Psychiatric Hospital, Zurich, Switzerland
- Intramural Research Program, Genetic Epidemiology Research Branch, NIMH, Bethesda, Md.
- Department of Psychiatry, University Hospital Center and University of Lausanne, Lausanne, Switzerland
- he Department of Psychiatry, University Hospital of Geneva, Geneva, Switzerland |
| Abstract |
Objective: There is growing clinical and epidemiologic evidence that major mood disorders form a spectrum from major depressive disorder to pure mania. The authors examined the prevalence and clinical correlates of major depressive disorder with subthreshold bipolarity compared with pure major depressive disorder in the National Comorbidity Survey Replication (NCS-R). Method: The NCS-R is a nationally representative face-to-face household survey of the U.S. population conducted between February 2001 and April 2003. Lifetime history of mood disorders, symptoms, and clinical indicators of severity were collected using version 3.0 of the World Health Organization's Composite International Diagnostic Interview. Results: Nearly 40% of study participants with a history of major depressive disorder had a history of subthreshold hypomania. This subgroup had a younger age at onset, more episodes of depression, and higher rates of comorbidity than those without a history of hypomania and lower levels of clinical severity than those with bipolar II disorder. Conclusions: These findings demonstrate heterogeneity in major depressive disorder and support the validity of inclusion of subthreshold mania in the diagnostic classification. The broadening of criteria for bipolar disorder would have important implications for research andclinical practice. |
| Principal Publication |
oui |
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Deckers R, Moonen CT. Ultrasound triggered, image guided, local drug delivery.
J Control Release. 2010 Aug 12 . 2010
original article. IF : 5.949
Liens vers résumé
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| Lab |
Imagerie moléculaire et fonctionnelle : de la physiologie à la thérapie - Chrit Moonen |
| Team |
Thérapies guidées par imagerie et Imagerie moléculaire - Chrit Moonen |
| European Collaboration |
-Image Sciences Institute, University Medical Center Utrecht, Heidelberglaan 100, Q0S.459, 3584 CX Utrecht, The Netherlands |
| Abstract |
Ultrasound allows the deposition of thermal and mechanical energies deep inside the human body in a non-invasive way. Ultrasound can be focused within a region with a diameter of about 1mm. The bio-effects of ultrasound can lead to local tissue heating, cavitation, and radiation force, which can be used for 1) local drug release from nanocarriers circulating in the blood, 2) increased extravasation of drugs and/or carriers, and 3) enhanced diffusivity of drugs. When using nanocarriers sensitive to mechanical forces (the oscillating ultrasound pressure waves) and/or sensitive to temperature, the content of the nanocarriers can be released locally. Thermo-sensitive liposomes have been suggested for local drug release in combination with local hyperthermia more than 25years ago. Microbubbles may be designed specifically to enhance cavitation effects. Real-time imaging methods, such as magnetic resonance, optical and ultrasound imaging have led to novel insights and methods for ultrasound triggered drug delivery. Image guidance of ultrasound can be used for: 1) target identification and characterization; 2) spatio-temporal guidance of actions to release or activate the drugs and/or permeabilize membranes; 3) evaluation of bio-distribution, pharmacokinetics and pharmacodynamics; and 4) physiological read-outs to evaluate the therapeutic efficacy. |
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de Senneville BD, Roujol S, Moonen C, Ries M. Motion correction in MR thermometry of abdominal organs: A comparison of the referenceless vs. the multibaseline approach. Magn Reson Med. 2010 Jul 30. 2010
original article. IF : 3.225
Liens vers résumé
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| Lab |
Imagerie moléculaire et fonctionnelle : de la physiologie à la thérapie - Chrit Moonen |
| Team |
Thérapies guidées par imagerie et Imagerie moléculaire - Chrit Moonen |
| Abstract |
Reliable temperature and thermal-dose measurements using proton resonance frequency shift-based magnetic resonance (MR) thermometry for MR-guided ablation of abdominal organs require a robust correction of artefacts induced by the target displacement through an inhomogeneous and time-variant magnetic field. Two correction approaches emerged recently as promising candidates to allow continuous real-time MR-thermometry under free-breathing conditions: The multibaseline correction method, which relies on a pre-recorded correction table allowing to correct for periodic phase changes, and the referenceless method, which depends on a background phase estimation in the target area based on the assumption of a smooth spatial variation of the phase across the organ. This study combines both methods with real-time in-plane motion correction to permit both temperature and thermal-dose calculations on the fly. Subsequently, the practical aspects of both methods are compared in two application scenarios, a radio frequency-ablation and a high-intensity focused ultrasound ablation. A hybrid approach is presented that exploits the strong points of both methods, allowing accurate and precise proton resonance frequency-thermometry measurements during periodical displacement, even in the presence of spontaneous motion and strong susceptibility variations in the target area. |
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de Oliveira PL, de Senneville BD, Dragonu I, Moonen CT. Rapid motion correction in MR-guided high-intensity focused ultrasound heating using real-time ultrasound echo information. NMR Biomed. 2010 Jul 28. 2010
Liens vers résumé
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| Lab |
Imagerie moléculaire et fonctionnelle : de la physiologie à la thérapie - Chrit Moonen |
| Team |
Thérapies guidées par imagerie et Imagerie moléculaire - Chrit Moonen |
| Abstract |
The objective of this study was to evaluate the feasibility of integrating real-time ultrasound echo guidance in MR-guided high-intensity focused ultrasound (HIFU) heating of mobile targets in order to reduce latency between displacement analysis and HIFU treatment. Experiments on a moving phantom were carried out with MRI-guided HIFU during continuous one-dimensional ultrasound echo detection using separate HIFU and ultrasound imaging transducers. Excellent correspondence was found between MR- and ultrasound-detected displacements. Real-time ultrasound echo-based target tracking during MR-guided HIFU heating is shown with the dimensions of the heated area similar to those obtained for a static target. This work demonstrates that the combination of the two modalities opens up perspectives for motion correction in MRI-guided HIFU with negligible latency. |
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Versini M, Jeandel PY, Fuzibet JG, Ianessi A, Hauger O, Amoretti N. Erdheim-Chester disease: Radiological findings.
Presse Med. 2010 Jul 23 . 2010
original article. IF : 0.416
Liens vers résumé
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| Lab |
Imagerie moléculaire et fonctionnelle : de la physiologie à la thérapie - Chrit Moonen |
| Team |
Thérapies guidées par imagerie et Imagerie moléculaire - Chrit Moonen |
| Abstract |
CONTEXT: Erdheim-Chester disease is a rare non-Langerhans form of histiocytosis. For the past years, the disease has been reported with an increasing frequency, linked to a better knowledge of it's radiological pattern. Indead, it shows specific imaging appearances, that should be recognized.
METHODS: We report four cases illustrating those typical imaging findings.
RESULTS: Common X-rays films show bilateral and symmetric heterogeneous osteosclerosis of the metaphysis and the diaphysis in the lower limbs long bones, with paget's disease-like pattern. Magnetic resonance imaging depicts a replacement of the normal fatty bone marrow by a heterogeneous high intensity signal infiltrate on T1 fat-suppressed weighted imaging with intravenous injection of gadolinium and T2 fat-suppressed weighted sequences, sparing the subchondral bone. Bone scintigraphy reveals a pathognomonic bilateral and symmetric increased uptake affecting both diaphysis and metaphysis of the femur and the tibiae. Tomodensitometry enable to disclose visceral and vascular involvement, showing typical "hairy kidney" appearance and perivascular infitration.
CONCLUSION: Erdheim-Chester disease may be a life-threatening disease. A good knowledge of it specific imaging features seems to be crucial for early management and improved prognosis. |
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Moreau MM, Piguel N, Papouin T, Koehl M, Durand CM, Rubio ME, Loll F, Richard EM, Mazzocco C, Racca C, Oliet SH, Abrous DN, Montcouquiol M, Sans N. The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function.
1Molecular and Cellular Neurobiology Group, 2Glia-Neuron Interactions Group, 3Neurogenesis and Pathophysiology Group, and 4Developmental Neurosciences Group, INSERM, Neurocentre Magendie, Laboratory of "Pathophysiology of Neural Plasticity," J Neurosci. 2010 Jul 21;30(29):9738-52. 2010
original article. IF : 7.178
Liens vers résumé
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| Lab |
Physiopathologie de la plasticité neuronale - Pier Vincenzo Piazza |
| Team |
Neurobiologie cellulaire et moléculaire - Equipe AVENIR - Nathalie Sans ; Neurogenèse et physiopathologie - D. Nora Abrous ; Neurosciences du développement - Equipe AVENIR - Mireille Montcouquiol ; Relations glie-neurone - Stéphane Oliet |
| Program areas |
Vulnérabilité, Toxicomanie |
| European Collaboration |
- Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
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| International Collaboration |
- Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269 USA
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| Abstract |
Scribble (Scrib) is a key regulator of apicobasal polarity, presynaptic architecture, and short-term synaptic plasticity in Drosophila. In mammals, its homolog Scrib1 has been implicated in cancer, neural tube closure, and planar cell polarity (PCP), but its specific role in the developing and adult nervous system is unclear. Here, we used the circletail mutant, a mouse model for PCP defects, to show that Scrib1 is located in spines where it influences actin cytoskeleton and spine morphing. In the hippocampus of these mutants, we observed an increased synapse pruning associated with an increased number of enlarged spines and postsynaptic density, and a decreased number of perforated synapses. This phenotype was associated with a mislocalization of the signaling pathway downstream of Scrib1, leading to an overall activation of Rac1 and defects in actin dynamic reorganization. Finally, Scrib1-deficient mice exhibit enhanced learning and memory abilities and impaired social behavior, two features relevant to autistic spectrum disorders. Our data identify Scrib1 as a crucial regulator of brain development and spine morphology, and suggest that Scrib1(crc/+) mice might be a model for studying synaptic dysfunction and human psychiatric disorders. |
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Marrs WR, Blankman JL, Horne EA, Thomazeau A, Lin YH, Coy J, Bodor AL, Muccioli GG, Hu SS, Woodruff G, Fung S, Lafourcade M, Alexander JP, Long JZ, Li W, Xu C, Möller T, Mackie K, Manzoni OJ, Cravatt BF, Stella N. The serine hydrolase ABHD6 controls the accumulation and efficacy of 2-AG at cannabinoid receptors. Nat Neurosci. 2010 Aug;13(8):951-7. 2010
Liens vers résumé
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| Lab |
Physiopathologie de la plasticité neuronale - Pier Vincenzo Piazza |
| European Collaboration |
- Bioanalysis and Pharmacology of Bioactive Lipids Laboratory, CHAM7230, Louvain Drug Research Institute, Université catholique de Louvain, Bruxelles, Belgium. |
| International Collaboration |
- Neurobiology and Behavior Graduate Program, University of Washington, Seattle, Washington, USA.
- The Skaggs Institute for Chemical Biology and Department of Chemical - Physiology, The Scripps Research Institute, La Jolla, California, USA.Department of - Pharmacology, University of Washington, Seattle, Washington, USA.
- Department of Otolaryngology, University of Washington, Seattle, Washington, USA.
Department of Psychological and Brain Sciences, Gill Center for Biomolecular Science, Indiana University, Bloomington, Indiana, USA.
- Neurobiology Undergraduate Program, University of Washington, Seattle,
- Department of Neurology, University of Washington, Seattle, Washington, USA.
- Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, Washington, USA. |
| Abstract |
The endocannabinoid 2-arachidonoylglycerol (2-AG) regulates neurotransmission and neuroinflammation by activating CB1 cannabinoid receptors on neurons and CB2 cannabinoid receptors on microglia. Enzymes that hydrolyze 2-AG, such as monoacylglycerol lipase, regulate the accumulation and efficacy of 2-AG at cannabinoid receptors. We found that the recently described serine hydrolase alpha-beta-hydrolase domain 6 (ABHD6) also controls the accumulation and efficacy of 2-AG at cannabinoid receptors. In cells from the BV-2 microglia cell line, ABHD6 knockdown reduced hydrolysis of 2-AG and increased the efficacy with which 2-AG can stimulate CB2-mediated cell migration. ABHD6 was expressed by neurons in primary culture and its inhibition led to activity-dependent accumulation of 2-AG. In adult mouse cortex, ABHD6 was located postsynaptically and its selective inhibition allowed the induction of CB1-dependent long-term depression by otherwise subthreshold stimulation. Our results indicate that ABHD6 is a rate-limiting step of 2-AG signaling and is therefore a bona fide member of the endocannabinoid signaling system. |
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